Phospho-Vimentin (Ser83) Rabbit mAb[0780]Cat NO.: A99040

Protein names ：VIM,VIME_HUMAN,Vimentin

UniProtID ：P08670

MASS(da) ：53,652

MW(kDa) ： 57

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser83 of human Vimentin

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HeLa cells treated with Paclitaxel(100 nM, G2/M arrested ,20 hr）.

Function ： Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.., Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2..

Tissue specificity ：Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines..

Subcellular locationi ：Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix. Cell membrane.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.