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14-3-3 beta/zeta Rabbit mAb[8JWU]Cat NO.: A91754

Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using 14-3-3 beta/zeta Rabbit mAb IgG [8JWU] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :YWHAB,1433B_HUMAN,14-3-3 protein beta/alpha [Cleaved into: 14-3-3 protein beta/alpha, N-terminally processed]

UniProtID :P31946/P63104

MASS(da) :27,745

MW(kDa) :28KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human 14-3-3 beta/zeta.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HT-29 cells

Function : Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13..

Subcellular locationi :Cytoplasm. Melanosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.