* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using 14-3-3 beta/zeta Rabbit mAb IgG [8JWU] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :YWHAB,1433B_HUMAN,14-3-3 protein beta/alpha [Cleaved into: 14-3-3 protein beta/alpha, N-terminally processed]
UniProtID :P31946/P63104
MASS(da) :27,745
MW(kDa) :28KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human 14-3-3 beta/zeta.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT-29 cells
Function : Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13..
Subcellular locationi :Cytoplasm. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.