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A-RAF Rabbit mAb[QAI8]Cat NO.: A32925

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using A-RAF Rabbit mAb IgG [QAI8] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :ARAF,ARAF1,PKS,PKS2,ARAF_HUMAN,Serine/threonine-protein kinase A-Raf

UniProtID :P10398

MASS(da) :67,585

MW(kDa) :68KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human A-RAF.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.., [Isoform 2]: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation..

Tissue specificity :Predominantly in urogenital tissues.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.