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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using AAMP Rabbit mAb IgG [2A17] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AAMP,AAMP_HUMAN,Angio-associated migratory cell protein
UniProtID :Q13685
MASS(da) :46,751
MW(kDa) :52KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human AAMP.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : Plays a role in angiogenesis and cell migration. In smooth muscle cell migration, may act through the RhoA pathway..
Tissue specificity :Expressed in metastatic melanoma, liver, skin, kidney, heart, lung, lymph node, skeletal muscle and brain, and also in A2058 melanoma cells and activated T-cells (at protein level). Expressed in blood vessels. Strongly expressed in endothelial cells, cytotrophoblasts, and poorly differentiated. colon adenocarcinoma cells found in lymphatics..
Subcellular locationi :Cell membrane. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.