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Western blot(SDS PAGE) analysis of extracts from Mouse lung tissue lysate.Using ACE1 Rabbit mAb IgG [34V9] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ACE,DCP,DCP1,ACE_HUMAN,Angiotensin-converting enzyme [Cleaved into: Angiotensin-converting enzyme, soluble form]
UniProtID :P12821
MASS(da) :149,715
MW(kDa) :195kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Mouse ACE1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse lung tissue lysate
Function : Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
Tissue specificity :Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis..
Subcellular locationi :[Angiotensin-converting enzyme, soluble form]: Secreted.,Cell membrane,Single-pass type I membrane protein. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.