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Western blot(SDS PAGE) analysis of extracts from C6 cells.Using ACHA4 Rabbit mAb IgG [TLLN] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CHRNA4,NACRA4,ACHA4_HUMAN,Neuronal acetylcholine receptor subunit alpha-4
UniProtID :P43681
MASS(da) :69,957
MW(kDa) :70kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ACHA4.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :C6 cells
Function : After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions..
Subcellular locationi :Cell junction, synapse, postsynaptic cell membrane,Multi-pass membrane protein. Cell membrane,Multi-pass membrane protein. Cell membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.