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ACHA4 Rabbit mAb[TLLN]Cat NO.: A30727

Western blot(SDS PAGE) analysis of extracts from C6 cells.Using ACHA4 Rabbit mAb IgG [TLLN] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CHRNA4,NACRA4,ACHA4_HUMAN,Neuronal acetylcholine receptor subunit alpha-4

UniProtID :P43681

MASS(da) :69,957

MW(kDa) :70kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ACHA4.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :C6 cells

Function : After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions..

Subcellular locationi :Cell junction, synapse, postsynaptic cell membrane,Multi-pass membrane protein. Cell membrane,Multi-pass membrane protein. Cell membrane,Lipid-anchor.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.