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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using ADRA1A Rabbit mAb IgG [0O02] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ADRA1A,ADRA1C,ADA1A_HUMAN,Alpha-1A adrenergic receptor
UniProtID :P35348
MASS(da) :51,487
MW(kDa) :58KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ADRA1A.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes..
Tissue specificity :Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart..
Subcellular locationi :Nucleus membrane,Multi-pass membrane protein. Cell membrane,Multi-pass membrane protein. Cytoplasm. Membrane, caveola.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.