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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using AdSS 2 Rabbit mAb IgG [T1VQ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ADSS2,ADSS,PURA2_HUMAN,Adenylosuccinate synthetase isozyme 2
UniProtID :P30520
MASS(da) :50,097
MW(kDa) :50KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human AdSS 2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP..
Subcellular locationi :Cytoplasm. Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.