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Western blot(SDS PAGE) analysis of extracts from .Using Aggrecan Rabbit mAb IgG [ZA41] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ACAN,AGC1,CSPG1,MSK16,PGCA_HUMAN,Aggrecan core protein [Cleaved into: Aggrecan core protein 2]
UniProtID :P16112
MASS(da) :261,329
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Aggrecan.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
Function : This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
Tissue specificity :Restricted to cartilages..
Subcellular locationi :Secreted, extracellular space, extracellular matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.