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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using AGL Rabbit mAb IgG [55LS] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AGL,GDE,GDE_HUMAN,Glycogen debranching enzyme [Includes: 4-alpha-glucanotransferase ,Amylo-alpha-1,6-glucosidase ]
UniProtID :P35573
MASS(da) :174,764
MW(kDa) :175KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human AGL.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.
Tissue specificity :Liver, kidney and lymphoblastoid cells express predominantly isoform 1,whereas muscle and heart express not only isoform 1, but also muscle-specific isoform mRNAs (isoforms 2, 3 and 4). Isoforms 5 and 6 are present in both liver and muscle.
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.