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Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using AHCY Rabbit mAb IgG [N5EL] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AHCY,SAHH,SAHH_HUMAN,Adenosylhomocysteinase
UniProtID :P23526
MASS(da) :47,716
MW(kDa) :47kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human AHCY.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT-29 cells
Function : Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine (PubMed:10933798). Binds copper ions (By similarity)..
Subcellular locationi :Cytoplasm. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.