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Western blot(SDS PAGE) analysis of extracts from K-562 cells.Using alpha 1 Spectrin Rabbit mAb IgG [4Z26] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SPTA1,SPTA,SPTA1_HUMAN,Spectrin alpha chain, erythrocytic 1
UniProtID :P02549
MASS(da) :280,014
MW(kDa) :280kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human alpha 1 Spectrin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K-562 cells
Function : Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
Subcellular locationi :Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.