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alpha 1 Spectrin Rabbit mAb[4Z26]Cat NO.: A30925

Western blot(SDS PAGE) analysis of extracts from K-562 cells.Using alpha 1 Spectrin Rabbit mAb IgG [4Z26] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :SPTA1,SPTA,SPTA1_HUMAN,Spectrin alpha chain, erythrocytic 1

UniProtID :P02549

MASS(da) :280,014

MW(kDa) :280kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human alpha 1 Spectrin.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :K-562 cells

Function : Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.

Subcellular locationi :Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.