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Western blot(SDS PAGE) analysis of extracts from Mouse brain tissue lysate.Using Alpha Fodrin Rabbit mAb IgG [3599] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SPTAN1,NEAS,SPTA2,SPTN1_HUMAN,Spectrin alpha chain, non-erythrocytic 1
UniProtID :Q13813
MASS(da) :284,539
MW(kDa) :170kDa/280kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Alpha Fodrin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse brain tissue lysate
Function : Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Subcellular locationi :Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.