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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using Alpha SNAP Rabbit mAb IgG [8WE2] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NAPA,SNAPA,SNAA_HUMAN,Alpha-soluble NSF attachment protein
UniProtID :P54920
MASS(da) :33,233
MW(kDa) :33kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Alpha SNAP.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus (Probable). Together with GNA12 promotes CDH5 localization to plasma membrane (PubMed:15980433)..
Subcellular locationi :Cell membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.