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ASS1 Rabbit mAb[9KS7]Cat NO.: A56554

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using ASS1 Rabbit mAb IgG [9KS7] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :ASS1,ASS,ASSY_HUMAN,Argininosuccinate synthase

UniProtID :P00966

MASS(da) :46,530

MW(kDa) :47KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ASS1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues..

Tissue specificity :Expressed in adult liver..

Subcellular locationi :Cytoplasm, cytosol.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.