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Western blot(SDS PAGE) analysis of extracts from Mouse heart tissue lysate.Using BNIP1 Rabbit mAb IgG [91XA] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BNIP1,NIP1,SEC20L,TRG8,SEC20_HUMAN,Vesicle transport protein SEC20
UniProtID :Q12981
MASS(da) :26,132
MW(kDa) :26KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human BNIP1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse heart tissue lysate
Function : As part of a SNARE complex may be involved in endoplasmic reticulum membranes fusion and be required for the maintenance of endoplasmic reticulum organization (PubMed:15272311). Plays also a role in apoptosis (PubMed:7954800, PubMed:15272311, PubMed:23896122). It is for instance required for endoplasmic reticulum stress-induced apoptosis (PubMed:23896122). As a substrate of RNF185 interacting with SQSTM1, might also be involved in mitochondrial autophagy (Probable)..
Tissue specificity :Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine..
Subcellular locationi :Endoplasmic reticulum membrane,Single-pass type IV membrane protein. Mitochondrion membrane,Single-pass type IV membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.