* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from NIH/3T3 cell Cobalt chloride - treated (0.1 mM).Using BNIP3 Rabbit mAb IgG [7V9I] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BNIP3,NIP3,BNIP3_HUMAN,BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
UniProtID :Q12983
MASS(da) :27,832
MW(kDa) :25/28kda
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human BNIP3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NIH/3T3 cell Cobalt chloride - treated (0.1 mM)
Function : Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway..
Subcellular locationi :Mitochondrion. Mitochondrion outer membrane,Single-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.