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BNIP3 Rabbit mAb[7V9I]Cat NO.: A52225

Western blot(SDS PAGE) analysis of extracts from NIH/3T3 cell Cobalt chloride - treated (0.1 mM).Using BNIP3 Rabbit mAb IgG [7V9I] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :BNIP3,NIP3,BNIP3_HUMAN,BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

UniProtID :Q12983

MASS(da) :27,832

MW(kDa) :25/28kda

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human BNIP3.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :NIH/3T3 cell Cobalt chloride - treated (0.1 mM)

Function : Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway..

Subcellular locationi :Mitochondrion. Mitochondrion outer membrane,Single-pass membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.