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Brachyury Rabbit mAb[KW59]Cat NO.: A12575

Western blot(SDS PAGE) analysis of extracts from NCI-H460 cells.Using Brachyury Rabbit mAb IgG [KW59] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :TBXT,T,TBXT_HUMAN,T-box transcription factor T

UniProtID :O15178

MASS(da) :47,443

MW(kDa) :50kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Brachyury.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :NCI-H460 cells

Function : Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site..

Tissue specificity :Detected in testis, but not in other, normal tissues. Detected in lung tumors (at protein level)..

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.