* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from NCI-H460 cells.Using Brachyury Rabbit mAb IgG [KW59] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TBXT,T,TBXT_HUMAN,T-box transcription factor T
UniProtID :O15178
MASS(da) :47,443
MW(kDa) :50kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Brachyury.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NCI-H460 cells
Function : Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site..
Tissue specificity :Detected in testis, but not in other, normal tissues. Detected in lung tumors (at protein level)..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.