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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Calpain 2 Rabbit mAb IgG [01BV] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CAPN2,CANPL2,CAN2_HUMAN,Calpain-2 catalytic subunit
UniProtID :P17655
MASS(da) :79,995
MW(kDa) :80kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Calpain 2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity)..
Tissue specificity :Ubiquitous.
Subcellular locationi :Cytoplasm. Cell membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.