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Calpain 2 Rabbit mAb[01BV]Cat NO.: A54564

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Calpain 2 Rabbit mAb IgG [01BV] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CAPN2,CANPL2,CAN2_HUMAN,Calpain-2 catalytic subunit

UniProtID :P17655

MASS(da) :79,995

MW(kDa) :80kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Calpain 2.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity)..

Tissue specificity :Ubiquitous.

Subcellular locationi :Cytoplasm. Cell membrane.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.