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Western blot(SDS PAGE) analysis of extracts from Mouse lung tissue lysate.Using Calponin Rabbit mAb IgG [65QI] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CNN1,CNN1_HUMAN,Calponin-1
UniProtID :P51911
MASS(da) :33,170
MW(kDa) :35KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Calponin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse lung tissue lysate
Function : Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity)..
Tissue specificity :Smooth muscle, and tissues containing significant amounts of smooth muscle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.