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Calponin Rabbit mAb[65QI]Cat NO.: A36522

Western blot(SDS PAGE) analysis of extracts from Mouse lung tissue lysate.Using Calponin Rabbit mAb IgG [65QI] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CNN1,CNN1_HUMAN,Calponin-1

UniProtID :P51911

MASS(da) :33,170

MW(kDa) :35KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Calponin.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Mouse lung tissue lysate

Function : Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity)..

Tissue specificity :Smooth muscle, and tissues containing significant amounts of smooth muscle.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.