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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using CAPG Rabbit mAb IgG [104A] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CAPG,AFCP,MCP,CAPG_HUMAN,Macrophage-capping protein
UniProtID :P40121
MASS(da) :38,499
MW(kDa) :38kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CAPG.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
Tissue specificity :Macrophages and macrophage-like cells.
Subcellular locationi :Nucleus. Cytoplasm. Melanosome. Cell projection, lamellipodium. Cell projection, ruffle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.