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Western blot(SDS PAGE) analysis of extracts from Mouse skeletal muscle tissue lysate.Using Cardiac troponin T (TNNT2) Rabbit mAb IgG [06CS] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TNNT2,TNNT2_HUMAN,Troponin T, cardiac muscle
UniProtID :P45379
MASS(da) :35,924
MW(kDa) :36KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Mouse Cardiac troponin T (TNNT2).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse skeletal muscle tissue lysate
Function : Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Tissue specificity :Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.