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Cardiac troponin T (TNNT2) Rabbit mAb[06CS]Cat NO.: A75269

Western blot(SDS PAGE) analysis of extracts from Mouse skeletal muscle tissue lysate.Using Cardiac troponin T (TNNT2) Rabbit mAb IgG [06CS] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :TNNT2,TNNT2_HUMAN,Troponin T, cardiac muscle

UniProtID :P45379

MASS(da) :35,924

MW(kDa) :36KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Mouse Cardiac troponin T (TNNT2).

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Mouse skeletal muscle tissue lysate

Function : Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Tissue specificity :Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.