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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using CARS Rabbit mAb IgG [FG3Z] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CARS1,CARS,SYCC_HUMAN,Cysteine--tRNA ligase, cytoplasmic
UniProtID :P49589
MASS(da) :85,473
MW(kDa) :85KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CARS.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys)..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.