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CARS Rabbit mAb[FG3Z]Cat NO.: A48892

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using CARS Rabbit mAb IgG [FG3Z] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CARS1,CARS,SYCC_HUMAN,Cysteine--tRNA ligase, cytoplasmic

UniProtID :P49589

MASS(da) :85,473

MW(kDa) :85KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CARS.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys)..

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.