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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Casein Kinase 2 beta (CSNK2B) Rabbit mAb IgG [70P9] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CSNK2B,CK2N,G5A,CSK2B_HUMAN,Casein kinase II subunit beta
UniProtID :P67870
MASS(da) :24,942
MW(kDa) :25kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Casein Kinase 2 beta (CSNK2B).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine (PubMed:11239457, PubMed:16818610). Participates in Wnt signaling (By similarity)..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.