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Western blot(SDS PAGE) analysis of extracts from Raji cells.Using CD23 Rabbit mAb IgG [LR0K] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :FCER2,CD23A,CLEC4J,FCE2,IGEBF,FCER2_HUMAN,Low affinity immunoglobulin epsilon Fc receptor [Cleaved into: Low affinity immunoglobulin epsilon Fc receptor membrane-bound form,Low affinity immunoglobulin epsilon Fc receptor soluble form]
UniProtID :P06734
MASS(da) :36,469
MW(kDa) :45KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CD23.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Raji cells
Function : Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
Subcellular locationi :Cell membrane,Single-pass type II membrane protein. Cell membrane,Lipid-anchor. Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.