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Western blot(SDS PAGE) analysis of extracts from BxPC-3 cells.Using CD239/BCAM Rabbit mAb IgG [89PJ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BCAM,LU,MSK19,BCAM_HUMAN,Basal cell adhesion molecule
UniProtID :P50895
MASS(da) :67,405
MW(kDa) :85KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CD239/BCAM.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :BxPC-3 cells
Function : Laminin alpha-5 receptor. May mediate intracellular signaling..
Tissue specificity :Wide tissue distribution (highest in the pancreas and very low in brain). Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.
Subcellular locationi :Membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.