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Western blot(SDS PAGE) analysis of extracts from THP-1 cells.Using CD27 Rabbit mAb IgG [53WN] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CD27,TNFRSF7,CD27_HUMAN,CD27 antigen
UniProtID :P26842
MASS(da) :29,137
MW(kDa) :55KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CD27.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :THP-1 cells
Function : Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Tissue specificity :Found in most T-lymphocytes.
Subcellular locationi :Membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.