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CD27 Rabbit mAb[53WN]Cat NO.: A96095

Western blot(SDS PAGE) analysis of extracts from THP-1 cells.Using CD27 Rabbit mAb IgG [53WN] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CD27,TNFRSF7,CD27_HUMAN,CD27 antigen

UniProtID :P26842

MASS(da) :29,137

MW(kDa) :55KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CD27.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :THP-1 cells

Function : Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Tissue specificity :Found in most T-lymphocytes.

Subcellular locationi :Membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.