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Western blot(SDS PAGE) analysis of extracts from NIH/3T3 cells.Using FaTTy Acid Synthase Rabbit pAb at dilution of 1:1000 incubated at 4℃ over night.
Protein names :FASN,FAS,FAS_HUMAN,Fatty acid synthase [Includes: [Acyl-carrier-protein] S-acetyltransferase ,[Acyl-carrier-protein] S-malonyltransferase ,3-oxoacyl-[acyl-carrier-protein] synthase ,3-oxoacyl-[acyl-carrier-protein] reductase ,3-hydroxyacyl-[acyl-carrier
UniProtID :P49327
MASS(da) :273,427
MW(kDa) :273 kDa
Form :Liquid
Purification :peptide affinity chromatography
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human FaTTy Acid Synthase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NIH/3T3 cells
Function : Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4
Tissue specificity :Ubiquitous. Prominent expression in brain, lung, liver and mammary gland..
Subcellular locationi :Cytoplasm. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.