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Western blot(SDS PAGE) analysis of extracts from Rat thymus tissue lysate.Using CD86 Rabbit mAb IgG [7CI1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CD86,CD28LG2,CD86_HUMAN,T-lymphocyte activation antigen CD86
UniProtID :P42081
MASS(da) :37,682
MW(kDa) :80kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CD86.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Rat thymus tissue lysate
Function : Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).., [Isoform 2]: Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.., (Microbial infection) Acts as a receptor for adenovirus subgroup B..
Tissue specificity :Expressed by activated B-lymphocytes and monocytes.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.