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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using CDC40 Rabbit mAb IgG [LBHO] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CDC40,EHB3,PRP17,PRPF17,PRP17_HUMAN,Pre-mRNA-processing factor 17
UniProtID :O60508
MASS(da) :65,521
MW(kDa) :75KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CDC40.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Required for pre-mRNA splicing as component of the activated spliceosome (PubMed:33220177). Plays an important role in embryonic brain development,this function does not require proline isomerization (PubMed:33220177)..
Subcellular locationi :Nucleus. Nucleus speckle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.