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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using CDKN2C/p18-INK4C Rabbit mAb IgG [H17U] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CDKN2C,CDKN6,CDN2C_HUMAN,Cyclin-dependent kinase 4 inhibitor C
UniProtID :P42773
MASS(da) :18,127
MW(kDa) :17KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CDKN2C/p18-INK4C.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.
Tissue specificity :Highest levels found in skeletal muscle. Also found in pancreas and heart.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.