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CHAT Rabbit mAb[OKI3]Cat NO.: A78059

Western blot(SDS PAGE) analysis of extracts from SH-SY5Y cells.Using CHAT Rabbit mAb IgG [OKI3] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CHAT,CLAT_HUMAN,Choline O-acetyltransferase

UniProtID :P28329

MASS(da) :82,536

MW(kDa) :65kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CHAT.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :SH-SY5Y cells

Function : Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses..

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.