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Western blot(SDS PAGE) analysis of extracts from SH-SY5Y cells.Using CHAT Rabbit mAb IgG [OKI3] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CHAT,CLAT_HUMAN,Choline O-acetyltransferase
UniProtID :P28329
MASS(da) :82,536
MW(kDa) :65kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CHAT.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SH-SY5Y cells
Function : Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.