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Western blot(SDS PAGE) analysis of extracts from NIH/3T3 cells.Using Clathrin heavy chain Rabbit mAb IgG [N6A9] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CLTC,CLH17,CLTCL2,KIAA0034,CLH1_HUMAN,Clathrin heavy chain 1
UniProtID :Q00610/P53675
MASS(da) :187,030
MW(kDa) :191kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Clathrin heavy chain.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NIH/3T3 cells
Function : Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582). The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825). Plays a role in early autophagosome formation (PubMed:20639872)..
Subcellular locationi :Cytoplasmic vesicle membrane,Peripheral membrane protein,Cytoplasmic side. Membrane, coated pit,Peripheral membrane protein,Cytoplasmic side. Melanosome. Cytoplasm, cytoskeleton, spindle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.