Collagen XVII/COL17A1 Rabbit mAb[1DEN]Cat NO.: A83636

Protein names ：COL17A1,BP180,BPAG2,COHA1_HUMAN,Collagen alpha-1 chain [Cleaved into: 120 kDa linear IgA disease antigen ,97 kDa linear IgA disease antigen ]

UniProtID ：Q9UMD9

MASS(da) ：150,419

MW(kDa) ：180KDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000,
• 免疫组化(IHC)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of human Collagen XVII/COL17A1.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：A-431 cells

Function ： May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane., The 120 kDa linear IgA disease antigen is an anchoring filament component involved in dermal-epidermal cohesion. Is the target of linear IgA bullous dermatosis autoantibodies.

Tissue specificity ：Detected in skin (PubMed:8618013). In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells (at protein level) (PubMed:25676728). Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves..

Subcellular locationi ：Cell junction, hemidesmosome. Membrane,Single-pass type II membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.