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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using COMT Rabbit mAb IgG [V514] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :COMT,COMT_HUMAN,Catechol O-methyltransferase
UniProtID :P21964
MASS(da) :30,037
MW(kDa) :25kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human COMT.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol..
Tissue specificity :Brain, liver, placenta, lymphocytes and erythrocytes.
Subcellular locationi :[Isoform Soluble]: Cytoplasm.,[Isoform Membrane-bound]: Cell membrane,Single-pass type II membrane protein,Extracellular side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.