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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using CPS1 Rabbit mAb IgG [T9X8] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CPS1,CPSM_HUMAN,Carbamoyl-phosphate synthase [ammonia], mitochondrial
UniProtID :P31327
MASS(da) :164,939
MW(kDa) :160kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CPS1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
Tissue specificity :Primarily in the liver and small intestine.
Subcellular locationi :Mitochondrion. Nucleus, nucleolus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.