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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using CPT2 Rabbit mAb IgG [R94E] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CPT2,CPT1,CPT2_HUMAN,Carnitine O-palmitoyltransferase 2, mitochondrial
UniProtID :P23786
MASS(da) :73,777
MW(kDa) :70kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CPT2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Involved in the intramitochondrial synthesis of acylcarnitines from accumulated acyl-CoA metabolites (PubMed:20538056, PubMed:24780397). Reconverts acylcarnitines back into the respective acyl-CoA esters that can then undergo beta-oxidation, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Active with medium (C8-C12) and long-chain (C14-C18) acyl-CoA esters (PubMed:20538056)..
Subcellular locationi :Mitochondrion inner membrane,Peripheral membrane protein,Matrix side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.