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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using CRMP2/DPYSL2 Rabbit mAb IgG [I90E] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DPYSL2,CRMP2,ULIP2,DPYL2_HUMAN,Dihydropyrimidinase-related protein 2
UniProtID :Q16555
MASS(da) :62,294
MW(kDa) :62KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CRMP2/DPYSL2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis..
Tissue specificity :Ubiquitous.
Subcellular locationi :Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.