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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using CYB5R3 Rabbit mAb IgG [5HT5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CYB5R3,DIA1,NB5R3_HUMAN,NADH-cytochrome b5 reductase 3 [Cleaved into: NADH-cytochrome b5 reductase 3 membrane-bound form,NADH-cytochrome b5 reductase 3 soluble form]
UniProtID :P00387
MASS(da) :34,235
MW(kDa) :35KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CYB5R3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Transfers two electrons from NADH to two molecules of cytochrome b5 through an enzyme-bound FAD. Electrons from the reduced cytochrome b5 are then transferred to various electron acceptors, thereby participating in methemoglobin reduction in red blood cells and in the desaturation and elongation of fatty acids, cholesterol biosynthesis and cytochrome P-450-mediated drug metabolism other tissues..
Tissue specificity :Isoform 2 is expressed at late stages of erythroid maturation.
Subcellular locationi :[Isoform 1]: Endoplasmic reticulum membrane,Lipid-anchor,Cytoplasmic side. Mitochondrion outer membrane,Lipid-anchor,Cytoplasmic side.,[Isoform 2]: Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.