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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using CYPOR Rabbit mAb IgG [PHQC] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :POR,CYPOR,NCPR_HUMAN,NADPH--cytochrome P450 reductase
UniProtID :P16435
MASS(da) :76,690
MW(kDa) :77kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CYPOR.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5..
Subcellular locationi :Endoplasmic reticulum membrane,Single-pass membrane protein,Cytoplasmic side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.