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Western blot(SDS PAGE) analysis of extracts from U-87MG cells.Using Cystatin B Rabbit mAb IgG [X617] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CSTB,CST6,STFB,CYTB_HUMAN,Cystatin-B
UniProtID :P04080
MASS(da) :11,140
MW(kDa) :13KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Cystatin B.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U-87MG cells
Function : This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.