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Western blot(SDS PAGE) analysis of extracts from Mouse brain tissue lysate.Using Cytokeratin 1 (KRT1) Rabbit mAb IgG [VS3R] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KRT1,KRTA,K2C1_HUMAN,Keratin, type II cytoskeletal 1
UniProtID :P04264
MASS(da) :66,039
MW(kDa) :70kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Cytokeratin 1 (KRT1).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse brain tissue lysate
Function : May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK..
Tissue specificity :The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
Subcellular locationi :Cell membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.