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Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using Cytokeratin 20 (KRT20) Rabbit mAb IgG [ALRJ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KRT20,K1C20_HUMAN,Keratin, type I cytoskeletal 20
UniProtID :P35900
MASS(da) :48,487
MW(kDa) :44kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Cytokeratin 20 (KRT20).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT-29 cells
Function : Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity)..
Tissue specificity :Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa,specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.