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Cytokeratin 20 (KRT20) Rabbit mAb[ALRJ]Cat NO.: A36574

Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using Cytokeratin 20 (KRT20) Rabbit mAb IgG [ALRJ] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :KRT20,K1C20_HUMAN,Keratin, type I cytoskeletal 20

UniProtID :P35900

MASS(da) :48,487

MW(kDa) :44kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Cytokeratin 20 (KRT20).

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HT-29 cells

Function : Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity)..

Tissue specificity :Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa,specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa..

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.