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Western blot(SDS PAGE) analysis of extracts from Mouse brain tissue lysate.Using DCAMKL1/DCLK1 Rabbit mAb IgG [58LY] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DCLK1,DCAMKL1,DCDC3A,KIAA0369,DCLK1_HUMAN,Serine/threonine-protein kinase DCLK1
UniProtID :O15075
MASS(da) :82,224
MW(kDa) :82kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human DCAMKL1/DCLK1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse brain tissue lysate
Function : Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
Tissue specificity :In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.