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Western blot(SDS PAGE) analysis of extracts from A-549 cells.Using Delta-sarcoglycan (SGCD) Rabbit mAb IgG [8525] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SGCD,SGCD_HUMAN,Delta-sarcoglycan
UniProtID :Q92629
MASS(da) :32,071
MW(kDa) :35kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Delta-sarcoglycan (SGCD).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A-549 cells
Function : Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Tissue specificity :Most strongly expressed in skeletal and cardiac muscle. Also detected in smooth muscle. Weak expression in brain and lung.
Subcellular locationi :Cell membrane, sarcolemma,Single-pass type II membrane protein. Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.