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DNA Ligase I Rabbit mAb[UZM0]Cat NO.: A66868

Western blot(SDS PAGE) analysis of extracts from Jurkat cells.Using DNA Ligase I Rabbit mAb IgG [UZM0] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :LIG1,DNLI1_HUMAN,DNA ligase 1

UniProtID :P18858

MASS(da) :101,736

MW(kDa) :130KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human DNA Ligase I.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Jurkat cells

Function : DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.