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Western blot(SDS PAGE) analysis of extracts from Jurkat cells.Using DNA Ligase I Rabbit mAb IgG [UZM0] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :LIG1,DNLI1_HUMAN,DNA ligase 1
UniProtID :P18858
MASS(da) :101,736
MW(kDa) :130KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human DNA Ligase I.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cells
Function : DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.