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Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using DNA Polymerase gamma Rabbit mAb IgG [I1TP] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :POLG,MDP1,POLG1,POLGA,DPOG1_HUMAN,DNA polymerase subunit gamma-1
UniProtID :P54098
MASS(da) :139,562
MW(kDa) :140kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human DNA Polymerase gamma.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT-29 cells
Function : Involved in the replication of mitochondrial DNA. Associates with mitochondrial DNA.
Subcellular locationi :Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.