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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Dopamine Receptor D3 (DRD3) Rabbit mAb IgG [HJ0F] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DRD3,DRD3_HUMAN,D dopamine receptor
UniProtID :P35462
MASS(da) :44,195
MW(kDa) :50kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Dopamine Receptor D3 (DRD3).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation..
Tissue specificity :Brain.
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.