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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using DTYMK Rabbit mAb IgG [MRB6] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DTYMK,CDC8,TMPK,TYMK,KTHY_HUMAN,Thymidylate kinase
UniProtID :P23919
MASS(da) :23,819
MW(kDa) :24KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human DTYMK.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Catalyzes the phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP), the immediate precursor for the DNA building block dTTP, with ATP as the preferred phosphoryl donor in the presence of Mg(2+)..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.