* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using DYNLL1 Rabbit mAb IgG [IXHI] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DYNLL1,DLC1,DNCL1,DNCLC1,HDLC1,DYL1_HUMAN,Dynein light chain 1, cytoplasmic
UniProtID :P63167
MASS(da) :10,366
MW(kDa) :10KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human DYNLL1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures., Binds and inhibits the catalytic activity of neuronal nitric oxide synthase., Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1.., Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity..
Tissue specificity :Ubiquitous (PubMed:8628263). Expressed in testis (PubMed:22965910)..
Subcellular locationi :Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton. Nucleus. Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.